Anticorps Polyclonal de lapin anti-DNA-PKcs

DNA-PKcs Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 19983-1-AP

Synonymes

DNA PK catalytic subunit, DNAPK, DNA-PKcs, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7

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Galerie de données de validation

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  WB analysis of HeLa using 19983-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 19983-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19983-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19983-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19983-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19983-1-AP (DNA-PKcs antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19983-1-AP

  IP result of anti-DNA-PKcs (IP:19983-1-AP, 4ug; Detection:19983-1-AP 1:500) with HeLa cells lysate 1320 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

19983-1-AP cible DNA-PKcs dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, DNA-activated, catalytic polypeptide
• Masse moléculaire calculée: 469 kDa
• Poids moléculaire observé: 350-460 kDa
• Numéro d’acquisition GenBank: NM_006904
• Symbole du gène: PRKDC
• Identification du gène (NCBI): 5591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.

Publications

Species	Application	Title
human	WB	Science; IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV.; Authors - Kathryn M Monroe
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
human	IF, WB	J Cell Biol; DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.; Authors - Shuyun Ma
human	WB	Int J Biol Macromol; Antitumor efficacy of a recombinant EGFR-targeted fusion protein conjugate that induces telomere shortening and telomerase downregulation; Authors - Hong-Yu Tao
human	WB,CoIP	Cancer Lett; ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer; Authors - Chen Zhou
human	WB	Cell Biol Toxicol; Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway.; Authors - Ruixue Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Strong correct bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart