Anticorps Polyclonal de lapin anti-pan-PKC

• Phare
• Validé par KD/KO

pan-PKC Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA, IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

22

Avis (2)

N° de cat : 12919-1-AP

Synonymes

PKC beta, PRKCB, PKC-beta, PKC-B, PKCB

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Galerie de données de validation

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  WB analysis using 12919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12919-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12919-1-AP

  IP result of anti-pan-PKC (IP:12919-1-AP, 4ug; Detection:12919-1-AP 1:2000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of human breast cancer using 12919-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12919-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of zebrafish retina using 12919-1-AP

  Immunofluorescence analysis of bipolar cells labelled with pan-PKC polyclonal antibody (12919-1-AP, green) on tissue sections of the zebrafish retina at 5 days post fertilisation. Dilution 1:300. Tissue fixed overnight in 4% PFA. Müller glia, red; DAPI, blue; HuC/D, magenta. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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  FC experiment of K-562 using 12919-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PKC Beta (12919-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules K-562, plaquettes de sang périphérique humain, tissu cérébral de rat, tissu cérébral de souris, tissu de thymus de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: zebrafish retina,
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

12919-1-AP cible pan-PKC dans les applications de WB, IHC, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

• Réactivité: Humain, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: pan-PKC Protéine recombinante Ag3591
• Nom complet: protein kinase C, beta
• Masse moléculaire calculée: 673 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC036472
• Symbole du gène: PRKCB
• Identification du gène (NCBI): 5579
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

Publications

Species	Application	Title
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
rat	WB	Am J Respir Cell Mol Biol; Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling.; Authors - Jessica B Snow ; KD Validated
human	WB	J Cell Mol Med; Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway.; Authors - Luo Wenjuan W
human	WB	Oxid Med Cell Longev; p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.; Authors - Ming Zhang
human	WB	J Cell Mol Med; Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.; Authors - Jing Cui
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ashish (Verified Customer) (05-21-2021)

This antibody gave very clear staining results in lung tissue. Very nice antibody to work.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Lung

pan-PKC Antibody Immunohistochemistry validation (1:300 dilution) in Lung (Cat no:12919-1-AP)

FH

Amy (Verified Customer) (09-06-2019)

This antibody dilution gave very clear protein bands in all murine colon samples used to date. Following ECL addition, the imager took ~240 seconds to detect PKCbeta (automatic exposure). Very easy antibody to work with

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Murine Colon Samples