Anticorps Polyclonal de lapin anti-PKC Alpha

• Phare
• Validé par KD/KO

PKC Alpha Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

47

Avis (4)

N° de cat : 21991-1-AP

Synonymes

PRKCA, PKCα, PKC-A, PKCA, PKC A

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Galerie de données de validation

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  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC Alpha antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21991-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21991-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 21991-1-AP

  IP result of anti-PKC Alpha (IP:21991-1-AP, 4ug; Detection:21991-1-AP 1:3000) with NIH/3T3 cells lysate 1320 ug.

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  IHC staining of human thyroid cancer using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 21991-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 21991-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 21991-1-AP (PKC alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21991-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (21991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules NIH/3T3, plaquettes de sang périphérique humain, tissu rénal de rat
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 37 publications below
• IHC: See 3 publications below
• IF: See 11 publications below

Informations sur le produit

21991-1-AP cible PKC Alpha dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Alpha Protéine recombinante Ag17275
• Nom complet: protein kinase C, alpha
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: AK055431
• Symbole du gène: PKC Alpha
• Identification du gène (NCBI): 5578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Publications

Species	Application	Title
human	WB	Theranostics; MED13L integrates Mediator-regulated epigenetic control into lung cancer radiosensitivity.; Authors - Nasha Zhang
human	IF	Cell Death Dis; ATF4/CEMIP/PKCα promotes anoikis resistance by enhancing protective autophagy in prostate cancer cells.; Authors - Ying Yu
human	WB	Cell Death Differ; β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.; Authors - Jia Ma
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Oncogenesis; Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth; Authors - Chunming Gu
human	WB	Front Pharmacol; Cellular mechanism of action of forsythiaside for the treatment of diabetic kidney disease; Authors - Chunmei Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (11-07-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence

FH

Chiara (Verified Customer) (10-04-2021)

Works very well in Immunoblot

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mesenchymal Stem cell

FH

Samantha (Verified Customer) (02-24-2020)

Bovine vascular smooth muscle cells were transfected with either control siRNA or PKCalpha siRNA for up to 7 days, with transfections repeated every 48-72 hours. Blocking agent: 5% (w/v) milk in TBST for 1hrPrimary antibody diluted in 5% (w/v) milk in TBST; incubated overnight at 4 degrees.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: Bovine vascular smooth muscle cells

PKC Alpha Antibody Western Blot, validation (1:750 dilution) in Bovine vascular smooth muscle cells (Cat no:21991-1-AP)

FH

Ryan (Verified Customer) (01-10-2018)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish Retina

PKC Alpha Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:21991-1-AP)