Anticorps Polyclonal de lapin anti-AMPK Beta 1

• Phare
• Validé par KD/KO

AMPK Beta 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10308-1-AP

Synonymes

PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1

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Galerie de données de validation

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  WB analysis of mouse liver using 10308-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10308-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10308-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10308-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10308-1-AP

  IP result of anti-AMPK Beta 1 (IP:10308-1-AP, 4ug; Detection:10308-1-AP 1:5000) with mouse liver tissue lysate 1960 ug.

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  IHC staining of human lung cancer using 10308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10308-1-AP (AMPK beta 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10308-1-AP (AMPK beta 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10308-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AMPK Beta 1 antibody (10308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A431, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10308-1-AP cible AMPK Beta 1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMPK Beta 1 Protéine recombinante Ag0301
• Nom complet: protein kinase, AMP-activated, beta 1 non-catalytic subunit
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001007
• Symbole du gène: PRKAB1
• Identification du gène (NCBI): 5564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880).

Publications

Species	Application	Title
human	WB,IP	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	Acta Pharm Sin B; Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy; Authors - Chang Xiao
mouse	WB,IF	Theranostics; Therapeutic inhibition of miR-802 protects against obesity through AMPK-mediated regulation of hepatic lipid metabolism.; Authors - Yangyue Ni
human,mouse	WB	J Ethnopharmacol; Mulberry leaves attenuate D-galactose-induced aging in vivo and in vitro; Authors - Yan Zhu
human	WB	Hum Cell; RNF126 contributes to stem cell-like properties and metastasis in hepatocellular carcinoma through ubiquitination and degradation of LKB1; Authors - Jie Huang
rat	WB	Dig Dis Sci; Heme Oxygenase-1-Modified BMMSCs Activate AMPK-Nrf2-FTH1 to Reduce Severe Steatotic Liver Ischemia-Reperfusion Injury; Authors - Xiaorong Tian