Anticorps Polyclonal de lapin anti-PRIM1

PRIM1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10773-1-AP

Synonymes

DNA primase 49 kDa subunit, DNA primase small subunit, p49, PRIM1

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Galerie de données de validation

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  WB analysis using 10773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Sp2/0 using 10773-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10773-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10773-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10773-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 10773-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10773-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10773-1-AP (PRIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10773-1-AP

  IP result of anti-PRIM1 (IP:10773-1-AP, 4ug; Detection:10773-1-AP 1:300) with HeLa cells lysate 2800ug.

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  IHC staining of human lymphoma using 10773-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10773-1-AP (PRIM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human thyroid cancer using 10773-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10773-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10773-1-AP (PRIM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10773-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRIM1 antibody (10773-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules Sp2/0
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

10773-1-AP cible PRIM1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRIM1 Protéine recombinante Ag1124
• Nom complet: primase, DNA, polypeptide 1 (49kDa)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC005266
• Symbole du gène: PRIM1
• Identification du gène (NCBI): 5557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA replication in human cells is initiated by a complex apparatus containing a DNA polymerase-alpha/primase complex. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. PRIM1 is a subnuit of this complex.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; 53BP1-shieldin-dependent DSB processing in BRCA1-deficient cells requires CST-Polα-primase fill-in synthesis.; Authors - Zachary Mirman
human	WB	Nucleic Acids Res; CST does not evict elongating telomerase but prevents initiation by ssDNA binding.; Authors - Arthur J Zaug
mouse		Cell Rep; A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice.; Authors - Liam C Hunt
human	WB	DNA Repair (Amst); Expression of BRCA1, BRCA2, RAD51, and other DSB repair factors is regulated by CRL4WDR70.; Authors - Zachary Mirman
	IHC	PLoS One; Optimization of the chicken chorioallantoic membrane assay as reliable in vivo model for the analysis of osteosarcoma.; Authors - Pierre Kunz
human	WB	bioRxiv; RTF2 controls replication repriming and ribonucleotide excision at the replisome; Authors - Brooke A Conti