Anticorps Polyclonal de lapin anti-PRDX4

• Phare
• Validé par KD/KO

PRDX4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 10703-1-AP

Synonymes

Peroxiredoxin IV, peroxiredoxin 4, EC:1.11.1.24, AOE37-2, AOE37 2

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Galerie de données de validation

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  WB analysis using 10703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10703-1-AP (PRDX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10703-1-AP

  WB result of PRDX4 antibody (10703-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX4 transfected HepG2 cells.

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  IP experiment of HepG2 using 10703-1-AP

  IP result of anti-PRDX4 (IP:10703-1-AP, 4ug; Detection:10703-1-AP 1:4000) with HepG2 cells lysate 2040 ug.

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  IHC staining of human colon cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10703-1-AP (PRDX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10703-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10703-1-AP (PRDX4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10703-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRDX4 antibody (10703-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10703-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PRDX4 (10703-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• IP: See 2 publications below

Informations sur le produit

10703-1-AP cible PRDX4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRDX4 Protéine recombinante Ag1177
• Nom complet: peroxiredoxin 4
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC007107
• Symbole du gène: PRDX4
• Identification du gène (NCBI): 10549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRDX4 (Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage (PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies (PMID:19208552). PRDX4 is a pentamer of dimers (PMID:23025503). This antibody is specific to PRDX4.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; The deubiquitinase OTUD1 regulates immunoglobulin production and proteasome inhibitor sensitivity in multiple myeloma; Authors - Alexander Vdovin
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB	Antioxid Redox Signal; Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.; Authors - Xiao-Jing Shi
human	WB	Antioxid Redox Signal; Direct Proteomic Mapping of Cysteine Persulfidation.; Authors - Ling Fu
mouse	WB	Bioorg Chem; Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents.; Authors - Chenhao Xu
	WB,IHC,IF,IP	FEBS J; A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems.; Authors - Daria Sicari