Anticorps Monoclonal anti-PRAME

• Phare
• Validé par KD/KO

PRAME Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1E9G9

N° de cat : 68097-1-Ig

Synonymes

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

---

Galerie de données de validation

• 

  WB analysis using 68097-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68097-1-Ig

  WB result of PRAME antibody (68097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRAME transfected HeLa cells.

• 

  WB analysis of HeLa using 68097-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 68097-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 68097-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 68097-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 68097-1-Ig (PRAME antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HaCaT using 68097-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody (68097-1-Ig, Clone: 1E9G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules A375, cellules HeLa, cellules K-562, cellules U2OS, tissu testiculaire de souris
• Résultats positifs en IF: cellules HaCaT,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68097-1-Ig cible PRAME dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRAME Protéine recombinante Ag1906
• Nom complet: preferentially expressed antigen in melanoma
• Masse moléculaire calculée: 509 aa, 58 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC014074
• Symbole du gène: PRAME
• Identification du gène (NCBI): 23532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.