Anticorps Polyclonal de lapin anti-PR repeat

PR repeat Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF, IHC, ELISA, Dot Blot

Conjugaison

Non conjugué

Publications

24

N° de cat : 23979-1-AP

Synonymes

C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP

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Galerie de données de validation

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  ELISA experiment of peptide using 23979-1-AP

  PR repeat Antibody (23979-1-AP) tested in ELISA. PR repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of PR repeat antibody (23979-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Applications testées

• Résultats positifs en ELISA: Peptide,

Dilution recommandée

• Test immuno-enzymatique (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 13 publications below

Informations sur le produit

23979-1-AP cible PR repeat dans les applications de WB, IF, IHC, ELISA, Dot Blot et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PR repeat
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.

Publications

Species	Application	Title
human	IHC	Nat Neurosci; RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.; Authors - Shizuka B Yamada
rat	WB, IF	Neuron; Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.; Authors - Xinmei Wen
mouse	IF	Nat Commun; Motor dysfunction and neurodegeneration in a C9orf72 mouse line expressing poly-PR.; Authors - Zongbing Hao
human	WB, IHC	Acta Neuropathol; Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.; Authors - Ian R A Mackenzie
human	WB	EMBO J; NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification.; Authors - Qing Tang
human	WB	EMBO J; The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.; Authors - Yuya Kawabe

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