Anticorps Polyclonal de lapin anti-PQBP1

PQBP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16264-1-AP

Synonymes

MRX55, MRXS3, MRXS8, PQBP 1, PQBP1, RENS1, SHS

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Galerie de données de validation

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  WB analysis using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16264-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16264-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16264-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16264-1-AP (PQBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16264-1-AP

  IP result of anti-PQBP1 (IP:16264-1-AP, 4ug; Detection:16264-1-AP 1:500) with mouse brain tissue lysate 3200 ug.

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  IHC staining of human breast cancer using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 16264-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16264-1-AP (PQBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16264-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16264-1-AP (PQBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon de rat, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16264-1-AP cible PQBP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PQBP1 Protéine recombinante Ag9329
• Nom complet: polyglutamine binding protein 1
• Masse moléculaire calculée: 265 aa, 30 kDa
• Poids moléculaire observé: 30-32 kDa
• Numéro d’acquisition GenBank: BC012358
• Symbole du gène: PQBP1
• Identification du gène (NCBI): 10084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PQBP1 (Polyglutamine-binding protein 1) is also named as NPW38 and JM26. PQBP1 senses extrinsic tau 3R/4R proteins by direct interaction and triggers an innate immune response by activating a cyclic GMP-AMP synthase (cGAS)-Stimulator of interferon genes (STING) pathway (PMID: 34782623).  PQBP1 is abundantly expressed in the central nervous system during development. PQBP1 is a component of the precatalytic spliceosome (B complex) that is involved in splicing. PQBP1 was predominantly localized in the cell nucleus. PQBP1 is overexpressed in various cancer types including breast cancer, colon cancer, and glioblastoma (PMID: 38342602).

Publications

Species	Application	Title
human	WB, IP, IF	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	mBio; Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.; Authors - Hongming Ma