Anticorps Polyclonal de lapin anti-PPP4C

PPP4C Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10262-1-AP

Synonymes

PP X, PP4, PP4C, PPH3, PPP4, PPP4C, PPX, Protein phosphatase X

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Galerie de données de validation

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  WB analysis of HEK-293 using 10262-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10262-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10262-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10262-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10262-1-AP

  IP result of anti-PPP4C (IP:10262-1-AP, 4ug; Detection:10262-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IF Staining of HEK-293 using 10262-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10262-1-AP (PPP4C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Informations sur le produit

10262-1-AP cible PPP4C dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP4C Protéine recombinante Ag0356
• Nom complet: protein phosphatase 4 (formerly X), catalytic subunit
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC001416
• Symbole du gène: PPP4C
• Identification du gène (NCBI): 5531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.

Publications

Species	Application	Title
	IP	Genes Dev; Protein phosphatase 4 controls circadian clock dynamics by modulating CLOCK/BMAL1 activity.; Authors - Sabrina Klemz
	WB,IF,IP	Cell Rep; Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression; Authors - Marisa Connell ; KD Validated
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
human	WB	FEBS Lett; Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6.; Authors - Chen Lu L
human	WB,IP	Cell Death Differ; AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner; Authors - Weimin Xu