Anticorps Polyclonal de lapin anti-PPP4C

PPP4C Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 10262-1-AP

Synonymes

PP X, PP4, PP4C, PPH3, PPP4, PPP4C, PPX, Protein phosphatase X



Applications testées

Résultats positifs en WBcellules HEK-293, cellules Jurkat, tissu rénal de souris
Résultats positifs en IPtissu rénal de souris
Résultats positifs en IF/ICCcellules HEK-293

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10262-1-AP cible PPP4C dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PPP4C Protéine recombinante Ag0356
Nom complet protein phosphatase 4 (formerly X), catalytic subunit
Masse moléculaire calculée 35 kDa
Poids moléculaire observé 35 kDa
Numéro d’acquisition GenBankBC001416
Symbole du gène PPP4C
Identification du gène (NCBI) 5531
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.

Protocole

Product Specific Protocols
WB protocol for PPP4C antibody 10262-1-APDownload protocol
IF protocol for PPP4C antibody 10262-1-APDownload protocol
IP protocol for PPP4C antibody 10262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Genes Dev

Protein phosphatase 4 controls circadian clock dynamics by modulating CLOCK/BMAL1 activity.

Authors - Sabrina Klemz
WB,IF,IP

Cell Rep

Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression

Authors - Marisa Connell
  • KD Validated
mouse

Front Cardiovasc Med

Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.

Authors - Yue Zheng
humanWB

FEBS Lett

Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6.

Authors - Chen Lu L
humanWB,IP

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu