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Anticorps Monoclonal anti-PPP2R2B

PPP2R2B Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E1D5

N° de cat : CL488-67783

Synonymes

B55 BETA, FLJ95686, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform beta, PP2A subunit B isoform R2 beta, PP2AB BETA, PP2APR55 BETA, PPP2R2B, PR2AB BETA, PR2AB55 BETA, PR2APR55 BETA, PR52B, PR55 BETA, SCA12

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PPP2R2B Monoclonal a (CL488-67783)

    FC experiment of HeLa using CL488-67783

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PPP2R2B (CL488-67783, Clone:2E1D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67783 cible PPP2R2B dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

Réactivité Humain, Lapin, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène PPP2R2B Protéine recombinante Ag30774
Nom complet protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
Masse moléculaire calculée 443 aa, 52 kDa
Poids moléculaire observé52 kDa
Numéro d’acquisition GenBankBC031790
Symbole du gène PPP2R2B
Identification du gène (NCBI) 5521
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing.

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 PPP2R2B antibody CL488-67783Download protocol
Standard Protocols
Click here to view our Standard Protocols