Anticorps Monoclonal anti-PPP2R2A/B/C

PPP2R2A/B/C Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2E1D5

N° de cat : 67783-1-Ig

Synonymes

B55 BETA, B55β, FLJ95686, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform beta, PP2A subunit B isoform R2 beta, PP2AB BETA, PP2APR55 BETA, PPP2R2A/B/C, PPP2R2B, PR2AB BETA, PR2AB55 BETA, PR2APR55 BETA, PR52B, PR55 BETA, SCA12

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Galerie de données de validation

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  WB analysis using 67783-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67783-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67783-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67783-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67783-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67783-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67783-1-Ig cible PPP2R2A/B/C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP2R2A/B/C Protéine recombinante Ag30774
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Masse moléculaire calculée: 443 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC031790
• Symbole du gène: PPP2R2B
• Identification du gène (NCBI): 5521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PPP2R2B gene encodes a deduced 443 amino acid protein of approximately 52 kDa, which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody can recognize PPP2R2A, PPP2R2B, and PPP2R2C due to the immunogen of PPP2R2B having high homology with PPP2R2A and PPP2R2C.

Publications

Species	Application	Title
human	WB	MedComm (2020); Regulation of XPO5 phosphorylation by PP2A in hepatocellular carcinoma.; Authors - Jiao Li