Anticorps Polyclonal de lapin anti-PPP1R8

PPP1R8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 16115-1-AP

Synonymes

NIPP1, NIPP 1, ARD-1, ARD1, ARD 1

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Galerie de données de validation

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  WB analysis of PC-3 using 16115-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16115-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16115-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16115-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16115-1-AP cible PPP1R8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP1R8 Protéine recombinante Ag9041
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 8
• Masse moléculaire calculée: 209 aa, 23 kDa
• Poids moléculaire observé: 47 kDa, 28 kDa, 19 kDa
• Numéro d’acquisition GenBank: BC013360
• Symbole du gène: PPP1R8
• Identification du gène (NCBI): 5511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.

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