Anticorps Polyclonal de lapin anti-PPP1R14B

PPP1R14B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 18476-1-AP

Synonymes

PHI 1, PLCB3N, PNG, PPP1R14B, SOM172

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Galerie de données de validation

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  WB analysis using 18476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18476-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 18476-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 18476-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18476-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18476-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 18476-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18476-1-AP (PPP1R14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules PC-3, cellules U2OS, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

18476-1-AP cible PPP1R14B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP1R14B Protéine recombinante Ag13373
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 14B
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC014522
• Symbole du gène: PPP1R14B
• Identification du gène (NCBI): 26472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse		Immunity; Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis.; Authors - Kiyoharu Fukushima
human	WB,IHC	J Cell Mol Med; PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma; Authors - Kang He