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Anticorps Polyclonal de lapin anti-PPP1R10

PPP1R10 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 24450-1-AP

Synonymes

CAT53, FB19, p99, PNUTS, PP1 binding protein of 114 kDa, PP1R10, PPP1R10, Protein FB19

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of HepG2 cells using PPP1R10 Polyclonal antibody (24450-1-AP)

    WB analysis of HepG2 using 24450-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24450-1-AP (PPP1R10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R10 Polyclonal antibody (24450-1-AP)

    IF Staining of HepG2 using 24450-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24450-1-AP (PPP1R10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBcellules HepG2
Résultats positifs en IF/ICCcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

24450-1-AP cible PPP1R10 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PPP1R10 Protéine recombinante Ag20091
Nom complet protein phosphatase 1, regulatory (inhibitor) subunit 10
Masse moléculaire calculée 940 aa, 99 kDa
Poids moléculaire observé 99 kDa
Numéro d’acquisition GenBankBC150310
Symbole du gène PPP1R10
Identification du gène (NCBI) 5514
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for PPP1R10 antibody 24450-1-APDownload protocol
IF protocol for PPP1R10 antibody 24450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Cell Biol

A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression.

Authors - Simon Grelet
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Roy (Verified Customer) (06-15-2024)

Perfect to detect PNUTS by WB (1/1000, Overnight incubation at 4°C) and by IF (1/250) in HeLa cells

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
FH

Fabienne (Verified Customer) (06-11-2024)

Works well in western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cell line