Anticorps Polyclonal de lapin anti-PDP1

PDP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 21176-1-AP

Synonymes

PDH, PDP, PDP 1, PDP1, PDPC, PDPC 1, PPM2C, Protein phosphatase 2C

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Galerie de données de validation

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  WB analysis using 21176-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21176-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21176-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21176-1-AP (PPM2C Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21176-1-AP

  IP result of anti-PDP1 (IP:21176-1-AP, 4ug; Detection:21176-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of human prostate cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 21176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21176-1-AP (PPM2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer de la thyroïde humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

21176-1-AP cible PDP1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDP1 Protéine recombinante Ag15453
• Nom complet: protein phosphatase 2C, magnesium-dependent, catalytic subunit
• Masse moléculaire calculée: 537 aa, 61 kDa
• Poids moléculaire observé: 55-61 kDa
• Numéro d’acquisition GenBank: BC047619
• Symbole du gène: PDP1
• Identification du gène (NCBI): 54704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pyruvate dehydrogenase phosphatase 1 (PDP1) is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

Publications

Species	Application	Title
human	IHC	Aging (Albany NY); Identification of immune-related cells and genes in the breast invasive carcinoma microenvironment.; Authors - Xingfeng Chen
human	WB	Curr Med Sci; Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway.; Authors - Jian-Sha Li
mouse	WB	J Diabetes Res; Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice.; Authors - Xiao Meng Zhang
human	WB	Cell Death Dis; Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling.; Authors - Yingying Shi
mouse	WB	ACS Chem Neurosci; FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke; Authors - Xin Guan
mouse	WB	J Physiol; Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle; Authors - Yuki Tamura