Anticorps Polyclonal de lapin anti-PPM1G

• Phare
• Validé par KD/KO

PPM1G Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15532-1-AP

Synonymes

PP2C gamma, PP2CG, PP2CGAMMA, PPM1C, PPM1G, PPP2CG, Protein phosphatase 1C, Protein phosphatase 1G

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Galerie de données de validation

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  WB analysis using 15532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15532-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 15532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 15532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Jurkat, cellules MCF-7, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu placentaire humain, tissu rénal humain, tissu splénique de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15532-1-AP cible PPM1G dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPM1G Protéine recombinante Ag7751
• Nom complet: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC000057
• Symbole du gène: PPM1G
• Identification du gène (NCBI): 5496
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Publications

Species	Application	Title
human	WB	Br J Pharmacol; Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.; Authors - Mao-Xu Ge
human	WB	J Oncol; Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development; Authors - Weiran Liao
human	WB,IHC	Cell Death Dis; PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.; Authors - Dawei Chen ; KD Validated