Anticorps Polyclonal de lapin anti-PPM1F

• Phare
• Validé par KD/KO

PPM1F Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17020-1-AP

Synonymes

Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM kinase phosphatase, CaM-kinase phosphatase, CaMKPase, EC:3.1.3.16

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Galerie de données de validation

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  WB analysis of MCF-7 using 17020-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17020-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17020-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17020-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17020-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17020-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 17020-1-AP

  IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.

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  IHC staining of human colon cancer using 17020-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17020-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17020-1-AP (PPM1F antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17020-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PPM1F antibody (17020-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules HepG2, cellules K-562, cellules L02, tissu hépatique humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: human ovary cancer tissue, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

17020-1-AP cible PPM1F dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPM1F Protéine recombinante Ag10186
• Nom complet: protein phosphatase 1F (PP2C domain containing)
• Masse moléculaire calculée: 453 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC071989
• Symbole du gène: PPM1F
• Identification du gène (NCBI): 9647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

Publications

Species	Application	Title
mouse	WB	J Cell Biol; PPM1F controls integrin activity via a conserved phospho-switch.; Authors - Tanja M Grimm ; KO Validated
human	WB	Clin Transl Oncol; PPM1F regulates ovarian cancer progression by affecting the dephosphorylation of ITGB1; Authors - Yahui Leng

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