Anticorps Polyclonal de lapin anti-PPM1A

PPM1A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications

3

N° de cat : 12961-1-AP

Synonymes

PP2C ALPHA, PP2CA, PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase IA

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Galerie de données de validation

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  WB analysis using 12961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12961-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12961-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules K-562, tissu hépatique humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12961-1-AP cible PPM1A dans les applications de WB, IF, IHC, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPM1A Protéine recombinante Ag3645
• Nom complet: protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC026691
• Symbole du gène: PPM1A
• Identification du gène (NCBI): 5494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPM1A(Protein phosphatase 1A) is also named as PPPM1A and belongs to the PP2C family. It is a serine/threonine protein phosphatase that is essential for regulating cellular stress responses in eukaryotes. PPM1A plays an important role in tumor suppression and that it negatively controls many signaling pathways which are critical for trophoblast invasion.(PMID:19404668). This protein distributes in both the cytoplasm and nucleus of mammalian cells(PMID:9003755). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	Cell treatment	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	WB	Biol Pharm Bull; Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes.; Authors - Aozora Nagaoka