Anticorps Polyclonal de lapin anti-PPARD

PPARD Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 10156-2-AP

Synonymes

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

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Galerie de données de validation

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  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10156-2-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10156-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10156-2-AP (PPARD antibody) at dilution of 1:10 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules COLO 320, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10156-2-AP cible PPARD dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPARD Protéine recombinante Ag0208
• Nom complet: peroxisome proliferator-activated receptor delta
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC002715
• Symbole du gène: PPARD
• Identification du gène (NCBI): 5467
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Publications

Species	Application	Title
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
		Diabetes; Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents.; Authors - Xinyu Yang
human	IHC	Cancer Lett; PMID: 32120025; Authors - Fangjia Tong
rat	WB	Eur J Med Chem; Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms.; Authors - Kai Liu
rat	WB	Neuropharmacology; PPARβ/δ activation protects against corticosterone-induced ER stress in astrocytes by inhibiting the CpG hypermethylation of microRNA-181a.; Authors - Juan Ji