Anticorps Polyclonal de lapin anti-BRN2-Specific

BRN2-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 18998-1-AP

Synonymes

BRN2, POU3F2, Brn-2, Brn 2, Brain-specific homeobox/POU domain protein 2

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Galerie de données de validation

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  WB analysis of HeLa using 18998-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18998-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18998-1-AP (BRN2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18998-1-AP (BRN2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18998-1-AP (BRN2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 18998-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human BRN2-Specific (18998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18998-1-AP cible BRN2-Specific dans les applications de WB, IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: POU class 3 homeobox 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: NM_005604
• Symbole du gène: BRN2
• Identification du gène (NCBI): 5454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

Publications

Species	Application	Title
human	WB	Genes Dev; Protein neddylation as a therapeutic target in pulmonary and extrapulmonary small cell carcinomas.; Authors - Justin P Norton
rat	WB	CNS Neurosci Ther; Reactive astrocytes targeting with oral vitamin A: Efficient neuronal regeneration for Parkinson's disease treatment and reversal of associated liver fibrosis; Authors - Nesrine Saeid El-Mezayen
	IF	Elife; Human-specific ARHGAP11B induces hallmarks of neocortical expansion in developing ferret neocortex.; Authors - Nereo Kalebic

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Etienne (Verified Customer) (12-19-2022)

Good antibody, worked best at 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoids

BRN2-Specific Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:18998-1-AP)