Anticorps Polyclonal de lapin anti-OCT2-Specific

• Phare
• Validé par KD/KO

OCT2-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18996-1-AP

Synonymes

OCT2, OCT2-Specific, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2

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Galerie de données de validation

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  WB analysis of mouse thymus using 18996-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18996-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18996-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 18996-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18996-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18996-1-AP (OCT2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 18996-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18996-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18996-1-AP (OCT2-Specific antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules Raji, tissu cérébral humain, tissu rénal de souris, tissu splénique humain
• Résultats positifs en IHC: tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

18996-1-AP cible OCT2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: POU class 2 homeobox 2
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 52-66 kDa
• Numéro d’acquisition GenBank: NM_002698
• Symbole du gène: POU2F2
• Identification du gène (NCBI): 5452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).

Publications

Species	Application	Title
human	WB	Mol Ther; miR-3687 Overexpression Promotes Bladder Cancer Cell Growth by Inhibiting the Negative Effect of FOXP1 on Cyclin E2 Transcription.; Authors - Qipeng Xie
human,mouse	WB,IHC	Int J Oncol; Receptor for activated C kinase 1 promotes cervical cancer lymph node metastasis via the glycolysis‑dependent AKT/mTOR signaling.; Authors - Lixiu Xu
human	WB	Biochem Biophys Res Commun; CBX7 represses the POU2F2 to inhibit the PD-L1 expression and regulate the immune response in bladder cancer.; Authors - Weigang Ren ; KD Validated
	WB	Toxicol In Vitro; Comparisons of segment-specific toxicity of platinum-based agents and cadmium using S1, S2, and S3 cells derived from mouse kidney proximal tubules.; Authors - Hitomi Fujishiro
mouse	WB	Age (Dordr); Aging increases the susceptibility of cisplatin-induced nephrotoxicity.; Authors - Jiagen Wen