Anticorps Polyclonal de lapin anti-POLR3D

POLR3D Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 15339-1-AP

Synonymes

BN51, BN51T, POLR3D, Protein BN51, RNA polymerase III subunit C4, RPC4, RPC53, RPC53 homolog, TSBN51

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Galerie de données de validation

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  WB analysis of HeLa using 15339-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15339-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15339-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15339-1-AP

  IP result of anti-POLR3D (IP:15339-1-AP, 4ug; Detection:15339-1-AP 1:300) with HeLa cells lysate 2600 ug.

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  IF Staining of HeLa using 15339-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15339-1-AP (POLR3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15339-1-AP cible POLR3D dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR3D Protéine recombinante Ag7542
• Nom complet: polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC004484
• Symbole du gène: POLR3D
• Identification du gène (NCBI): 661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.

Publications

Species	Application	Title
human	WB	FEBS J; Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.; Authors - Richa Ahuja