Anticorps Polyclonal de lapin anti-POLR1C

POLR1C Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 15923-1-AP

Synonymes

AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40

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Galerie de données de validation

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  WB analysis using 15923-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15923-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 15923-1-AP

  IP result of anti-POLR1C (IP:15923-1-AP, 4ug; Detection:15923-1-AP 1:800) with NIH/3T3 cells lysate 1600 ug.

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  IHC staining of human ovary using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 15923-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules Caco-2, cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu ovarien humain, tissu cardiaque humain, tissu cutané humain, tissu hépatique de souris, tissu hépatique humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15923-1-AP cible POLR1C dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR1C Protéine recombinante Ag8746
• Nom complet: polymerase (RNA) I polypeptide C, 30kDa
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC008863
• Symbole du gène: POLR1C
• Identification du gène (NCBI): 9533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLR1C is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

Publications

Species	Application	Title
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Krishnendu (Verified Customer) (02-27-2020)

Nice antibody. Got a specific band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T