Anticorps Polyclonal de lapin anti-POLQ

POLQ Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 28590-1-AP

Synonymes

POLH, EC:2.7.7.49, DNA polymerase theta, DNA polymerase eta

---

Galerie de données de validation

• 

  WB analysis using 28590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 28590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 28590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HuH-7, cellules L02
• Résultats positifs en IF/ICC: cellules A375, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28590-1-AP cible POLQ dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLQ Protéine recombinante Ag29643
• Nom complet: polymerase (DNA directed), theta
• Masse moléculaire calculée: 290 kDa
• Poids moléculaire observé: 250~290 kDa, 197 kDa
• Numéro d’acquisition GenBank: NM_199420
• Symbole du gène: POLQ
• Identification du gène (NCBI): 10721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).

Publications

Species	Application	Title
human,mouse	WB	Phytomedicine; Anemarchalconyn, a natural alkyne ketone compound, inhibits HCC cell growth by suppressing Polθ and inducing synthetic lethality in Homologous recombination deficiency cells; Authors - Junnan Li

{{ptg:RelatedPrimaryAntibodies}}