Anticorps Polyclonal de lapin anti-POLDIP2

POLDIP2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15080-1-AP

Synonymes

PDIP38, POLDIP2

---

Galerie de données de validation

• 

  WB analysis of HeLa using 15080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15080-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15080-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15080-1-AP (POLDIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15080-1-AP

  IP result of anti-POLDIP2 (IP:15080-1-AP, 4ug; Detection:15080-1-AP 1:1000) with HeLa cells lysate 1600ug.

• 

  IF Staining of HeLa using 15080-1-AP

  Immunofluorescent analysis of Hela cells, using POLDIP2 antibody 15080-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15080-1-AP cible POLDIP2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLDIP2 Protéine recombinante Ag7154
• Nom complet: polymerase (DNA-directed), delta interacting protein 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC000655
• Symbole du gène: POLDIP2
• Identification du gène (NCBI): 26073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass.; Authors - Maga Giovanni G
human	IF	Cell Cycle; PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2.; Authors - Agnes Wong
human,mouse	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key