Anticorps Polyclonal de lapin anti-DNA Polymerase Beta

• Phare
• Validé par KD/KO

DNA Polymerase Beta Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 18003-1-AP

Synonymes

DNA polymerase beta, POLB

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Galerie de données de validation

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  WB analysis using 18003-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18003-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18003-1-AP

  IP result of anti-DNA Polymerase Beta (IP:18003-1-AP, 4ug; Detection:18003-1-AP 1:800) with K-562 cells lysate 3200ug.

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  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18003-1-AP (DNA polymerase beta antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below

Informations sur le produit

18003-1-AP cible DNA Polymerase Beta dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNA Polymerase Beta Protéine recombinante Ag12496
• Nom complet: polymerase (DNA directed), beta
• Masse moléculaire calculée: 335 aa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC100288
• Symbole du gène: DNA Polymerase Beta
• Identification du gène (NCBI): 5423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human		Proc Natl Acad Sci U S A; Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription.; Authors - Shiladitya Sengupta
mouse	WB	Cancer Cell Int; Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines.; Authors - Yuanyuan Wang ; KO Validated
human	WB	DNA Repair (Amst); Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.; Authors - Shiladitya Sengupta
human		NAR Cancer; XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells.; Authors - Bradley J Eckelmann