Anticorps Polyclonal de lapin anti-PNPLA3

PNPLA3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11442-1-AP

Synonymes

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, iPLA(2)epsilon, iPLA2 epsilon, PNPLA3

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Galerie de données de validation

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  WB analysis using 11442-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11442-1-AP (PNPLA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11442-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11442-1-AP (PNPLA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11442-1-AP (PNPLA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11442-1-AP (PNPLA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules L02,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

11442-1-AP cible PNPLA3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PNPLA3 Protéine recombinante Ag1959
• Nom complet: patatin-like phospholipase domain containing 3
• Masse moléculaire calculée: 481 aa, 53 kDa
• Poids moléculaire observé: 50-70 kDa, 130-150 kDa
• Numéro d’acquisition GenBank: BC014449
• Symbole du gène: PNPLA3
• Identification du gène (NCBI): 80339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)

Publications

Species	Application	Title
mouse	WB	Diabetes; Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis Via Impairment of Muscle Lipid Uptake and Storage.; Authors - Qiaoli Chen
human,rat	WB	Arch Biochem Biophys; Baicalin ameliorates alcohol-induced hepatic steatosis by suppressing SREBP1c elicited PNPLA3 competitive binding to ATGL.; Authors - Pan Li
human	WB	Diabetes; N-end Rule-Mediated Proteasomal Degradation of ATGL Promotes Lipid Storage; Authors - Jiesi Xu