Anticorps Polyclonal de lapin anti-Pancreatic Lipase

Pancreatic Lipase Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11209-1-AP

Synonymes

Lipase, Pancreatic Lipase, PL, PNLIP, PTL

---

Galerie de données de validation

• 

  WB analysis of BxPC-3 using 11209-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11209-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11209-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:50.

---

Applications testées

• Résultats positifs en WB: cellules BxPC-3, tissu pancréatique de souris, tissu pancréatique humain
• Résultats positifs en IHC: tissu pancréatique de souris, tissu de cancer du pancréas humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

11209-1-AP cible Pancreatic Lipase dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pancreatic Lipase Protéine recombinante Ag1681
• Nom complet: pancreatic lipase
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC014309
• Symbole du gène: PNLIP
• Identification du gène (NCBI): 5406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The pancreatic triacylglycerol lipase precursor (PNLIP), a 56 kDa protein secreted by the pancreas that is essential for the hydrolysis and absorption of long-chain triglyceride fatty acids in the intestine is located ~1 Mb upstream from the chromosome 10 peak(PMID:17890784). It is also named as PL, PTL and belongs to the AB hydrolase superfamily. It may potentially be involved in the pathophysiology of traumatic brain injury and may be implicated in the proliferation of astrocytes and the recovery of neurological outcomes(PMID:19760487). The full length protein has a signal peptide with 16 amino acid and a glycosylation site.

Publications

Species	Application	Title
mouse	IHC	Int J Mol Sci; Blocking β₁/β₂-Adrenergic Signaling Reduces Dietary Fat Absorption by Suppressing Expression of Pancreatic Lipase in High Fat-Fed Mice.; Authors - Kyunghwa Baek
pig	WB	JPEN J Parenter Enteral Nutr; Early Enteral Administration of a Complex Lipid Emulsion Supplement Prevents Postnatal Deficits in Docosahexaenoic and Arachidonic Acids and Increases Tissue Accretion of Lipophilic Nutrients in Preterm Piglets.; Authors - Olajumoke Akinsulire
human	IHC	Am J Cancer Res; A human pan-cancer system analysis of heat shock protein family A member 5; Authors - Chao Deng