Anticorps Monoclonal anti-PMS1

PMS1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1A6G3

N° de cat : 68413-1-Ig

Synonymes

DKFZp781M0253, FLJ98259, HNPCC3, hPMS1, PMS1, PMS1 protein homolog 1, PMSL1

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Galerie de données de validation

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  WB analysis using 68413-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68413-1-Ig (PMS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of Caco-2 using 68413-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PMS1 antibody (68413-1-Ig, Clone: 1A6G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules K-562, cellules LNCaP, cellules MOLT-4
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

68413-1-Ig cible PMS1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PMS1 Protéine recombinante Ag28127
• Nom complet: PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
• Poids moléculaire observé: 106 kDa
• Numéro d’acquisition GenBank: BC096331
• Symbole du gène: PMS1
• Identification du gène (NCBI): 5378
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.

Publications

Species	Application	Title
human	WB	Nat Commun; Splice modulators target PMS1 to reduce somatic expansion of the Huntington's disease-associated CAG repeat; Authors - Zachariah L McLean

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