Anticorps Polyclonal de lapin anti-PMPCB

• Phare
• Validé par KD/KO

PMPCB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 16064-1-AP

Synonymes

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB

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Galerie de données de validation

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  WB analysis of MCF-7 using 16064-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16064-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16064-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16064-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16064-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 16064-1-AP

  IP result of anti-PMPCB (IP:16064-1-AP, 3ug; Detection:16064-1-AP 1:500) with MCF-7 cells lysate 2560ug.

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  IHC staining of human lung cancer using 16064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16064-1-AP (PMPCB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

16064-1-AP cible PMPCB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PMPCB Protéine recombinante Ag8937
• Nom complet: peptidase (mitochondrial processing) beta
• Masse moléculaire calculée: 489 aa, 54 kDa
• Poids moléculaire observé: 43-52 kDa
• Numéro d’acquisition GenBank: BC010398
• Symbole du gène: PMPCB
• Identification du gène (NCBI): 9512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

Publications

Species	Application	Title
human	WB	Nature; Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.; Authors - Xiaoyan Guo
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
human	WB	EMBO J; PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.; Authors - Shotaro Saita
	WB,IF	Autophagy; The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance.; Authors - Giulia Bertolin ; KD Validated
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian