Anticorps Polyclonal de lapin anti-PMM1

PMM1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11252-2-AP

Synonymes

phosphomannomutase 1, PMM 1, PMM1, PMMH 22, PMMH22, Sec53

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Galerie de données de validation

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  WB analysis of mouse brain using 11252-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11252-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11252-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11252-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11252-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11252-2-AP (PMM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11252-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11252-2-AP (PMM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, cellules NIH/3T3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11252-2-AP cible PMM1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PMM1 Protéine recombinante Ag1782
• Nom complet: phosphomannomutase 1
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC016818
• Symbole du gène: PMM1
• Identification du gène (NCBI): 5372
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PMM1, also named as PMMH22, belongs to the eukaryotic PMM family. It catalyzes the second step in the conversion of fructose-6P to GDP-mannoseis and is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, PMM1 may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain(PMID:18927083).

Publications

Species	Application	Title
mouse	WB	J Proteome Res; Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.; Authors - Wegrzynowicz Michal M