Anticorps Polyclonal de lapin anti-PML

• Phare
• Validé par KD/KO

PML Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 21041-1-AP

Synonymes

MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19

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Galerie de données de validation

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  WB analysis of HeLa using 21041-1-AP

  WB result of PML antibody (21041-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PML transfected HeLa cells.

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  WB analysis of HEK-293 using 21041-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21041-1-AP (PML Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21041-1-AP

  IP result of anti-PML (IP:21041-1-AP, 4ug; Detection:21041-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human liver using 21041-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21041-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21041-1-AP (PML antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

21041-1-AP cible PML dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PML Protéine recombinante Ag15377
• Nom complet: promyelocytic leukemia
• Masse moléculaire calculée: 882 aa, 98 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC020994
• Symbole du gène: PML
• Identification du gène (NCBI): 5371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	IHC,IF	Theranostics; Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.; Authors - Wen Li
human	WB	Front Oncol; Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.; Authors - Shengye Liu
human	WB,IF	J Ginseng Res; 20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades.; Authors - Sirui Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

The antibody works really well in ICC in human cells (in 5% goat serum in PBST, ON incubation).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, U2OS cells

PML Antibody Immunofluorescence validation (1:1000 dilution) in Human, U2OS cells (Cat no:21041-1-AP)

FH

Eishi (Verified Customer) (11-17-2017)

Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein

PML Antibody Western Blot validation (1:500 dilution) in Protein (Cat no:21041-1-AP)