Anticorps Polyclonal de lapin anti-PLOD2-Specific

• Phare
• Validé par KD/KO

PLOD2-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

48

Avis (1)

N° de cat : 21214-1-AP

Synonymes

LH2, Lysyl hydroxylase 2, PLOD2-Specific, TLH

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Galerie de données de validation

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  WB analysis of A431 using 21214-1-AP

  WB result of PLOD2-Specific antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.

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  WB analysis using 21214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21214-1-AP

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21214-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 21214-1-AP

  IP result of anti-PLOD2-Specific (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.

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  IHC staining of human liver cancer using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21214-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21214-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2-Specific antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21214-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2-Specific (21214-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules A431, cellules DU 145, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HT-1080
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de muscle squelettique humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 35 publications below
• IHC: See 19 publications below
• IF: See 6 publications below

Informations sur le produit

21214-1-AP cible PLOD2-Specific dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, Lapin, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLOD2-Specific Protéine recombinante Ag15614
• Nom complet: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
• Masse moléculaire calculée: 758 aa, 85 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC037169
• Symbole du gène: PLOD2
• Identification du gène (NCBI): 5352
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

Publications

Species	Application	Title
human	WB, IHC	J Clin Invest; Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.; Authors - Yulong Chen
zebrafish	WB	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
human, mouse	WB	Cancer Discov; Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.; Authors - Eisinger-Mathason T S Karin TS
mouse	WB	Arthritis Rheumatol; Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.; Authors - Akio Yamamoto
human	WB	Proc Natl Acad Sci U S A; Intratumoral oxygen gradients mediate sarcoma cell invasion.; Authors - Daniel M Lewis
mouse	IHC	Arthritis Rheum; Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.; Authors - Remst D F G DF

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RICHA (Verified Customer) (12-02-2019)

Excellent

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast