Anticorps Polyclonal de lapin anti-PLOD1

PLOD1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12475-1-AP

Synonymes

LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1

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Galerie de données de validation

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  WB analysis of Fibroblasts cells using 12475-1-AP

  WB result of PLOD1 antibody (12475-1-AP, 1:200) with Protein extracts from Human Fibroblasts.

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  WB analysis of Mouse Fibroblast cells using 12475-1-AP

  WB result of PLOD1 antibody (12475-1-AP, 1:250) with Osteoblasts/Fibroblasts/714 cell line Lysate.

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of Mouse skin papilloma using 12475-1-AP

  IHC result of PLOD1 antibody (12475-1-AP, 1:100) with mouse skin papilloma slides.

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Applications testées

• Résultats positifs en WB: cellules fibroblastes, cellules fibroblastes de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12475-1-AP cible PLOD1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLOD1 Protéine recombinante Ag3103
• Nom complet: procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
• Masse moléculaire calculée: 727 aa, 84 kDa
• Poids moléculaire observé: 85-88 kDa
• Numéro d’acquisition GenBank: BC016657
• Symbole du gène: PLOD1
• Identification du gène (NCBI): 5351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLOD1, also named as LLH, PLOD and LH1, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. PLOD1 catalyses the hydroxylation of lysine residues during the posttranslational modification of type I collagen, the major protein of bone.

Publications

Species	Application	Title
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB,IHC	Bone; Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.; Authors - Tomoaki Saito
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima
mouse	WB	J Pharmacol Exp Ther; Minoxidil Cannot Be Used To Target Lysyl Hydroxylases during Postnatal Mouse Lung Development: A Cautionary Note.; Authors - Tilman Pfeffer
Bumblebees	IF	Front Genet; Mating Stimulates the Immune Response and Sperm Storage-Related Genes Expression in Spermathecae of Bumblebee (Bombus terrestris) Queen.; Authors - Yueqin Guo