Anticorps Polyclonal de lapin anti-PLK4

• Phare
• Validé par KD/KO

PLK4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 12952-1-AP

Synonymes

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

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Galerie de données de validation

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  WB analysis of A549 cells using 12952-1-AP

  WB result of PLK4 antibody (12952-1-AP, 1:2000) with si-control and si-PLK4 transfected A549 cells.

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  WB analysis using 12952-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12952-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12952-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules A549, cellules MCF-7
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 10 publications below
• IF: See 6 publications below

Informations sur le produit

12952-1-AP cible PLK4 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLK4 Protéine recombinante Ag3605
• Nom complet: polo-like kinase 4 (Drosophila)
• Masse moléculaire calculée: 970 aa, 109 kDa
• Poids moléculaire observé: 105-112 kDa
• Numéro d’acquisition GenBank: BC036023
• Symbole du gène: PLK4
• Identification du gène (NCBI): 10733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse, human	WB, IHC	Nat Commun; Loss of KLF14 triggers centrosome amplification and tumorigenesis.; Authors - Guangjian Fan ; KO Validated
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human	WB,IHC	Cell Death Dis; Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.; Authors - Xiangdong Tian ; KD Validated
human	WB	Cell Death Dis; NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.; Authors - Min Li
human	WB	Mol Cancer Ther; Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo.; Authors - Ke Liu