Anticorps Polyclonal de lapin anti-PLK1

• Phare
• Validé par KD/KO

PLK1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 10305-1-AP

Synonymes

Polo-like kinase 1, Polo like kinase 1, PLK-1, PLK 1, PLK

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Galerie de données de validation

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  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 10305-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PLK1 antibody (10305-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of Ramos using 10305-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules Jurkat, cellules MDA-MB-453s, cellules U2OS, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

10305-1-AP cible PLK1 dans les applications de WB, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLK1 Protéine recombinante Ag0284
• Nom complet: polo-like kinase 1 (Drosophila)
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC002369
• Symbole du gène: PLK1
• Identification du gène (NCBI): 5347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

Publications

Species	Application	Title
human	WB,IF	J Clin Invest; PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models; Authors - Marta Baldrighi
human	WB	Adv Sci (Weinh); Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer; Authors - Huaiyuan Liang ; KD Validated
human,mouse	WB	Sci Adv; Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system; Authors - Nachuan Song
human	WB,IF	Nat Commun; Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.; Authors - Wengui Shi
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
rat	WB	Cell Rep; Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex; Authors - Kellen D Winden

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PF00011