Anticorps Polyclonal de lapin anti-tPA

• Phare
• Validé par KD/KO

tPA Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 10147-1-AP

Synonymes

Alteplase, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, Tissue Plasminogen Activator, tPA, t-Plasminogen activator

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Galerie de données de validation

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  WB analysis using 10147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10147-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10147-1-AP

  IP result of anti-tPA (IP:10147-1-AP, 3ug; Detection:10147-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse brain using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10147-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10147-1-AP (tPA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu pancréatique de souris, tissu rénal de rat
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Informations sur le produit

10147-1-AP cible tPA dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: tPA Protéine recombinante Ag0200
• Nom complet: plasminogen activator, tissue
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 32-35 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC002795
• Symbole du gène: tPA
• Identification du gène (NCBI): 5327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Redox Biol; FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.; Authors - Ying Cai ; KO Validated
mouse	IF	J Neurosci; Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile x mice.; Authors - V Swaroop Achuta
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma
mouse	IHC	Glia; Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures.; Authors - Ewa E Bres
human	WB	Sci Rep; Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.; Authors - Yang Liu
mouse	IF	Cell Cycle; tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.; Authors - Ling Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

nuo (Verified Customer) (08-22-2021)

There is a band at around 64 kD as expected, although there were other nonspecific bindings.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

tPA Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10147-1-AP)