Anticorps Polyclonal de lapin anti-PLAGL2

• Phare
• Validé par KD/KO

PLAGL2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11540-1-AP

Synonymes

KIAA0198, PLAGL2, Zinc finger protein PLAGL2

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Galerie de données de validation

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  WB analysis using 11540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11540-1-AP (PLAGL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11540-1-AP cible PLAGL2 dans les applications de WB, IP, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLAGL2 Protéine recombinante Ag2137
• Nom complet: pleiomorphic adenoma gene-like 2
• Masse moléculaire calculée: 496 aa, 55 kDa
• Poids moléculaire observé: 50-55 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC023655
• Symbole du gène: PLAGL2
• Identification du gène (NCBI): 5326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429).

Publications

Species	Application	Title
human	WB,IHC,IF	EBioMedicine; PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.; Authors - Daojiang Li ; KD Validated
human	IP	Cancers (Basel); Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.; Authors - Qinghua Wang
human,mouse	WB,IHC,IF	Carcinogenesis; PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis; Authors - Wen Chen
human	WB	Apoptosis; Pleomorphic adenoma gene-like 2 regulates expression of the p53 family member, p73, and induces cell cycle block and apoptosis in human promonocytic U937 cells.; Authors - Hanks Tracey S TS
human	WB,IHC	Pediatr Res; Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2.; Authors - Liang Wu ; KD Validated
human	WB,IF	Gene; Modulation of PLAGL2 transactivation by positive cofactor 2 (PC2), a component of the ARC/Mediator complex.; Authors - Wezensky Sara J SJ