• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-PAFAH

PAFAH Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 15526-1-AP

Synonymes

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, Lp-PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PAF-AH, PLA2G7



Applications testées

Résultats positifs en WBcellules RAW 264.7, RAW264.7, tissu hépatique de souris, tissu splénique de souris
Résultats positifs en IHCtissu d'amygdalite humain, tissu de cancer de la prostate humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules RAW 264.7,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15526-1-AP cible PAFAH dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PAFAH Protéine recombinante Ag7227
Nom complet phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Masse moléculaire calculée 50 kDa
Poids moléculaire observé 43-67 kDa
Numéro d’acquisition GenBankBC038452
Symbole du gène PLA2G7
Identification du gène (NCBI) 7941
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).

Protocole

Product Specific Protocols
WB protocol for PAFAH antibody 15526-1-APDownload protocol
IHC protocol for PAFAH antibody 15526-1-APDownload protocol
IF protocol for PAFAH antibody 15526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Authors - Das Amrita A
mouseWB

Nat Commun

VDAC2 enables BAX to mediate apoptosis and limit tumor development.

Authors - Hui San Chin
human,mouseIF,IHC,WB

J Immunother Cancer

Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinoma

Authors - Feng Zhang
mouseWB

J Extracell Vesicles

Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells.

Authors - Yuting Liang
mouseIF,WB

Nat Metab

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.

Authors - Jacob T Boyd
  • KD Validated
humanIF

Cell Death Discov

Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis.

Authors - Junyi Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sathyanarayani (Verified Customer) (05-12-2023)

I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Lysed mouse blood