- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-PAFAH
PAFAH Polyclonal Antibody for WB, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 15526-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules RAW 264.7, RAW264.7, tissu hépatique de souris, tissu splénique de souris |
Résultats positifs en IHC | tissu d'amygdalite humain, tissu de cancer de la prostate humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules RAW 264.7, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 2 publications below |
WB | See 8 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Informations sur le produit
15526-1-AP cible PAFAH dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | PAFAH Protéine recombinante Ag7227 |
Nom complet | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
Masse moléculaire calculée | 50 kDa |
Poids moléculaire observé | 43-67 kDa |
Numéro d’acquisition GenBank | BC038452 |
Symbole du gène | PLA2G7 |
Identification du gène (NCBI) | 7941 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
PAFAH(platelet-activating factor acetylhydrolase, plasma) is also named as PLA2G7(phospholipase A2, group VII), Lp-PLA2 (Lipoprotein-associated phospholipase A2), LDL-PLA2, LDL-PLA and belongs to the AB hydrolase superfamily and lipase family. It is an enzyme which catalyzes the hydrolysis of acetyl ester at the sn-2 position of PAF. The family of PAF-Ahs consists of two intracellular isoforms (Ib and II), and one secreted isoform (plasma). Plasma PAF-AH is a monomeric polypeptide with a molecular weight of 45 kDa and it can show broad bands with a molecular weight of 43-67 kDa that it can be extensively N-glycosylated(PMID:12547653). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PAFAD), susceptibility to asthma (ASTHMA), susceptibility to atopic hypersensitivity (ATOPY).
Protocole
Product Specific Protocols | |
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WB protocol for PAFAH antibody 15526-1-AP | Download protocol |
IHC protocol for PAFAH antibody 15526-1-AP | Download protocol |
IF protocol for PAFAH antibody 15526-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation. | ||
J Immunother Cancer Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinoma | ||
J Extracell Vesicles Characterization of protein, long noncoding RNA and microRNA signatures in extracellular vesicles derived from resting and degranulated mast cells. | ||
Nat Metab Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions.
| ||
Cell Death Discov Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sathyanarayani (Verified Customer) (05-12-2023) | I used this PAFAH antibody at a range of different dilutions from 1:300 to 1:1000 but unfortunately I could not detect any bands from this antibody when I used lysed mouse blood. The loading controls which were also from Proteintech produced strong clear bands on the western blot.
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