Anticorps Polyclonal de lapin anti-PKLR

• Phare
• Validé par KD/KO

PKLR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 22456-1-AP

Synonymes

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

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Galerie de données de validation

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  WB analysis of multi-cells/tissue using 22456-1-AP

  WB results of 22456-1-AP.

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  WB analysis using 22456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle tissue using 22456-1-AP

  WB result of 22456-1-AP. (anti-PKM2 and PKLR antibodies are used as negative controls.).

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  WB analysis of HEK-293 using 22456-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse muscle/liver tissue using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).

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  WB analysis of mouse heart tissue using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of 293 cell using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control).

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  WB analysis of MCF-7 using 22456-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 22456-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 22456-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22456-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2.MCF7 cells using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis using 22456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22456-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22456-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules 293, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules NIH/3T3, cellules Raji, multi-cellules/tissus, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique humain, tissu musculaire/hépatique de souris, tissu rénal humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22456-1-AP cible PKLR dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKLR Protéine recombinante Ag17933
• Nom complet: pyruvate kinase, liver and RBC
• Masse moléculaire calculée: 574 aa, 62 kDa
• Poids moléculaire observé: 58-62 kDa
• Numéro d’acquisition GenBank: BC025737
• Symbole du gène: PKLR
• Identification du gène (NCBI): 5313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

Publications

Species	Application	Title
human	WB	Nat Med; Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.; Authors - Weier Qi
rat	WB	Cell Metab; Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.; Authors - Abudukadier Abulizi
human	WB,IHC	Mol Ther; Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis.; Authors - Zhijun Liu
mouse	WB	PLoS Genet; PMID: 24831725; Authors - Yukimasa Takeda
mouse	WB	Int J Mol Sci; Loss of miR-23b/27b/24-1 Cluster Impairs Glucose Tolerance via Glycolysis Pathway in Mice.; Authors - Yong-Hui Jiang
human	WB,IHC	Sci Rep; Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.; Authors - Wei Lu