Anticorps Polyclonal de lapin anti-Polycystin 2

Polycystin 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19126-1-AP

Synonymes

APKD2, PC2, PKD2, PKD4, Polycystin 2, Polycystwin, R48321

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Galerie de données de validation

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  WB analysis using 19126-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19126-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19126-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19126-1-AP

  IP result of anti-Polycystin 2 (IP:19126-1-AP, 3ug; Detection:19126-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IHC staining of human kidney using 19126-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 19126-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 19126-1-AP

  Immunofluorescent analysis of MDCK cells using 19126-1-AP (Polycystin 2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu rénal humain
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

19126-1-AP cible Polycystin 2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: polycystic kidney disease 2 (autosomal dominant)
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 109 kDa
• Numéro d’acquisition GenBank: NM_000297
• Symbole du gène: PKD2
• Identification du gène (NCBI): 5311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)

Publications

Species	Application	Title
mouse	WB	Front Mol Biosci; The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries.; Authors - Xin Hou
human	IHC	Hum Pathol; Down-regulation of polycystin in lymphatic malformations: Possible role in the proliferation of lymphatic endothelial cells.; Authors - Jian-Gang Ren
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu
mouse	IF,IHC,WB	Graefes Arch Clin Exp Ophthalmol; TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells; Authors - Wenxu Zheng