Anticorps Monoclonal anti-PKM2-specific

• Phare
• Validé par KD/KO

PKM2-specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ICC, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

40

CloneNo.

1C11C7

Avis (1)

N° de cat : 60268-1-Ig

Synonymes

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

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Galerie de données de validation

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  WB analysis of HEK-293 using 60268-1-Ig

  WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells.

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  WB analysis using 60268-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of MCF-7 using 60268-1-Ig

  Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60268-1-Ig

  Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60268-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60268-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60268-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60268-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60268-1-Ig

  IP Result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug.

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of HeLa using 60268-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 60268-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  FC experiment of HepG2 using 60268-1-Ig

  1X10^6 HepG2 cells were stained with .2ug PKM2-specific antibody (60268-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules C6, cellules HepG2, cellules HSC-T6, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules ROS1728, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:30000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 34 publications below
• IHC: See 7 publications below
• IF: See 16 publications below
• IP: See 5 publications below
• CoIP: See 4 publications below

Informations sur le produit

60268-1-Ig cible PKM2-specific dans les applications de WB, IP, IHC, IF, ICC, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PKM pyruvate kinase, muscle
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_002654
• Symbole du gène: PKM
• Identification du gène (NCBI): 5315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
rat	WB,IF,ICC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu ; KD Validated
human	WB	Autophagy; SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.; Authors - Bin Zhang
rat	WB	Acta Physiol (Oxf); Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.; Authors - Lian Tian
human	WB,IHC,IF	Mol Ther Nucleic Acids; Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma; Authors - Bei Zhou ; KD Validated
mouse	WB	Int Immunopharmacol; Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice; Authors - Si-Yao Sang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, and incubated with secondary antibody AF647 at room temperature for 2 h.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Ovarian tissue

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:60268-1-Ig)

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