Anticorps Polyclonal de lapin anti-PITX1

• Phare
• Validé par KD/KO

PITX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10873-1-AP

Synonymes

BFT, Homeobox protein PITX1, paired like homeodomain 1, Pituitary homeobox 1, PITX1, POTX, PTX1

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Galerie de données de validation

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  WB analysis of HeLa using 10873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10873-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10873-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10873-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10873-1-AP

  IP result of anti-PITX1 (IP:10873-1-AP, 4ug; Detection:10873-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 10873-1-AP

  IP result of anti-PITX1 (IP:10873-1-AP, 4ug; Detection:10873-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IHC staining of human tonsillitis using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10873-1-AP

  Immunofluorescent analysis of HepG2 cells, using PITX1 antibody 10873-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10873-1-AP cible PITX1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PITX1 Protéine recombinante Ag1303
• Nom complet: paired-like homeodomain 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC009412
• Symbole du gène: PITX1
• Identification du gène (NCBI): 5307
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PITX1, also named as Pituitary homeobox 1 or BFT, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. Bicoid subfamily. PITX1 localizes in the nucleus and may play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
	WB,IF	Hum Mutat; Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability.; Authors - Godelieve Morel
mouse	WB	Endocrinology; Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice.; Authors - Hiraku Kameda
human	WB,IHC	Int J Biochem Cell Biol; Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.; Authors - Yu Jin ; KD Validated
rat	WB,IF	Toxicol Lett; All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression.; Authors - Wang Yun-Guo YG