Anticorps Polyclonal de lapin anti-PIP5K3

• Phare
• Validé par KD/KO

PIP5K3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA, Dot Blot

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 13361-1-AP

Synonymes

CFD, FAB1, KIAA0981, PIKFYVE, PIP5K, PIP5K3, PIPkin III, Type III PIP kinase

---

Galerie de données de validation

• 

  WB analysis of mouse lung using 13361-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13361-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13361-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13361-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

13361-1-AP cible PIP5K3 dans les applications de WB, IHC, ELISA, Dot Blot et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIP5K3 Protéine recombinante Ag4177
• Nom complet: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
• Masse moléculaire calculée: 451 aa, 50 kDa, 237 kDa
• Poids moléculaire observé: 237 kDa
• Numéro d’acquisition GenBank: BC032389
• Symbole du gène: PIP5K3
• Identification du gène (NCBI): 200576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIP5K3 (1-phosphatidylinositol 3-phosphate 5-kinase), also callled PIKFYVE, belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol (PMID: 15902656; 9858586). The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4 (PMID: 17556371). In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity (PubMed: 11714711; 17556371).PIP5K3 has four isoforms with molecular weights of 237 kDa, 50 kDa, 51 kDa and 62 kDa. This antibody primarily recognizes the 237 kDa isoform.

Publications

Species	Application	Title
		Mol Cell; TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.; Authors - Katharina Fitzian ; KD Validated
human	WB	Elife; Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.; Authors - Sai Srinivas Panapakkam Giridharan
pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su
human	IHC	Front Oncol; TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.; Authors - Jie Jiang
		Eur J Pharmacol; Inhibition of c-Rel DNA binding is critical for the anti-inflammatory effects of novel PIKfyve inhibitor.; Authors - Masaomi Terajima
mouse	Dot Blot	BMC Immunol; miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.; Authors - Jingsong Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kincaid (Verified Customer) (05-21-2024)

This antibody works very well. I use it 1:200 - overnight incubation at 4°C (3% BSA in TBST). I have validated with H4 cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: H4 cell

PIP5K3 Antibody Western Blot validation (1:200 dilution) in H4 cell (Cat no:13361-1-AP)