Anticorps Polyclonal de lapin anti-PIP4K2B

• Phare
• Validé par KD/KO

PIP4K2B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13218-1-AP

Synonymes

PI(5)P 4 kinase type II beta, PI5P4KB, PIP4K2B, PIP4KII beta, PIP5K2B, PIP5KIIB, PIP5KIIbeta

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Galerie de données de validation

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  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13218-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13218-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13218-1-AP (PIP4K2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13218-1-AP (PIP4K2B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13218-1-AP (PIP4K2B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas cancer using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13218-1-AP (PIP4K2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13218-1-AP (PIP4K2B Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

13218-1-AP cible PIP4K2B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIP4K2B Protéine recombinante Ag4099
• Nom complet: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• Masse moléculaire calculée: 416 aa, 47 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC027459
• Symbole du gène: PIP4K2B
• Identification du gène (NCBI): 8396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer. It binds TNFRSF1A(24/32/50kd).

Publications

Species	Application	Title
mouse	WB	Nat Commun; MANF regulates hypothalamic control of food intake and body weight.; Authors - Su Yang ; KD Validated
mouse	IHC	Dev Cell; PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.; Authors - Archna Ravi