Anticorps Polyclonal de lapin anti-PIP4K2A

• Phare
• Validé par KD/KO

PIP4K2A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12469-1-AP

Synonymes

PI(5)P 4 kinase type II alpha, PI5P4KA, PIP4K2A, PIP4KII alpha, PIP5K2, PIP5K2A, PIP5KII alpha, PIP5KIIA, PIP5KIII, PIPK, PtdIns(4)P 5 kinase B isoform, PtdIns(4)P 5 kinase C isoform

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Galerie de données de validation

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  WB analysis of mouse brain using 12469-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12469-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12469-1-AP

  IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IHC staining of human spleen using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 12469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu splénique humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

12469-1-AP cible PIP4K2A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIP4K2A Protéine recombinante Ag3152
• Nom complet: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• Masse moléculaire calculée: 406 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC018034
• Symbole du gène: PIP4K2A
• Identification du gène (NCBI): 5305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse,human	IF,IHC	Dev Cell; PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.; Authors - Archna Ravi
human	IF,IHC,IP,WB	Int J Biochem Cell Biol; A novel lncRNA lncSAMD11-1:1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization; Authors - Mei Zhang
human,mouse	WB,IHC,IF	Sci Adv; PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition; Authors - Joanna Triscott ; KD Validated