Anticorps Polyclonal de lapin anti-PINX1

• Phare
• Validé par KD/KO

PINX1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 12368-1-AP

Synonymes

LPTL, LPTS, PIN2 interacting protein 1, Pin2 interacting protein X1, PINX1, Protein 67 11 3, TRF1 interacting protein 1

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Galerie de données de validation

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  WB analysis using 12368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12368-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12368-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12368-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12368-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12368-1-AP (PINX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules C6, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 8 publications below

Informations sur le produit

12368-1-AP cible PINX1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PINX1 Protéine recombinante Ag3043
• Nom complet: PIN2-interacting protein 1
• Masse moléculaire calculée: 328 aa, 37 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC015479
• Symbole du gène: PINX1
• Identification du gène (NCBI): 54984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.

Publications

Species	Application	Title
human	WB	Oncogene; Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.; Authors - Zhang B B ; KD Validated
human	IHC	Mol Cancer; The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway.; Authors - Xiao-Peng Tian
human	WB,IHC	Am J Cancer Res; PINX1 promotes malignant transformation of thyroid cancer through the activation of the AKT/MAPK/β-catenin signaling pathway.; Authors - JiHoon Kang ; KD Validated
human	WB,IHC	Mol Cancer; PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway.; Authors - Liu Jian-Ye JY
human	WB,IHC	Cancer Lett; PMID: 25045845; Authors - Xiao-Peng Tian
human	WB,IHC	Cancer Sci; Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma.; Authors - Cai Mu-Yan MY