Anticorps Polyclonal de lapin anti-PIN1

• Phare
• Validé par KD/KO

PIN1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (1)

N° de cat : 10495-1-AP

Synonymes

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5

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Galerie de données de validation

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  WB analysis of HEK-293 using 10495-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10495-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10495-1-AP

  IP result of anti-PIN1 (IP:10495-1-AP, 4ug; Detection:10495-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human renal cell carcinoma using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10495-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10495-1-AP (PIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 10495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 31 publications below
• IHC: See 7 publications below
• IF: See 9 publications below
• IP: See 2 publications below

Informations sur le produit

10495-1-AP cible PIN1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIN1 Protéine recombinante Ag0767
• Nom complet: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC002899
• Symbole du gène: PIN1
• Identification du gène (NCBI): 5300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

Publications

Species	Application	Title
human	WB,IF,IP	Nat Commun; Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells; Authors - Qiuhong Zhu
human,mouse	WB	J Pineal Res; Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.; Authors - Dongmei Chen
human	WB,IF	J Hazard Mater; Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.; Authors - Fuli Zheng
human	WB	Theranostics; SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.; Authors - Ling Lin
human	WB	Nucleic Acids Res; Pin1 promotes GR transactivation by enhancing recruitment to target genes.; Authors - Poolman Toryn M TM ; KD Validated
mouse	WB, IHC, IF	Oxid Med Cell Longev; PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.; Authors - Yanzhuo Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other