Anticorps Polyclonal de lapin anti-VPS34 (C terminal)

• Phare
• Validé par KD/KO

VPS34 (C terminal) Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

55

N° de cat : 12452-1-AP

Synonymes

hVps34, PI 3 Kinase Class 3, PI 3-kinase p100, PI3K p100, PI3K type 3, PIK3C3, PtdIns 3 kinase type 3, VPS34, VPS34 (C terminal)

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Galerie de données de validation

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  WB analysis of Jurkat using 12452-1-AP

  WB result of VPS34 (C terminal) antibody (12452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 (C terminal) transfected Jurkat cells.

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  WB analysis of PC-3 using 12452-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12452-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12452-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12452-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12452-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12452-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12452-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12452-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12452-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12452-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12452-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12452-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12452-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12452-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12452-1-AP (VPS34 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 12452-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12452-1-AP (VPS34 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12452-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12452-1-AP (VPS34 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 12452-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using VPS34 (C terminal) antibody (12452-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu pulmonaire de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 52 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12452-1-AP cible VPS34 (C terminal) dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS34 (C terminal) Protéine recombinante Ag3110
• Nom complet: phosphoinositide-3-kinase, class 3
• Masse moléculaire calculée: 887 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC033004
• Symbole du gène: VPS34
• Identification du gène (NCBI): 5289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Publications

Species	Application	Title
mouse	WB,IF	J Thromb Haemost; Vps34 derived phosphatidylinositol 3-monophosphate modulates megakaryocyte maturation and proplatelet production through late endosomes/lysosomes.; Authors - Ivana Bertović
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
human	WB	Nat Commun; Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.; Authors - Shirley Tremel
	IP,CoIP	Nat Commun; IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.; Authors - Fuqing Hu
	WB	Autophagy; Modification of BECN1 by ISG15 Plays a Crucial Role in Autophagy Regulation by Type I IFN/interferon.; Authors - Daichao Xu